P-063 Microfluidic technology is highly effective in selecting a sperm population with high progressive motility and low DNA fragmentation index

Abstract Study question Which sperm preparation procedure is able to select the best population of sperm? Summary answer Microfluidic sorting using Fertile Plus device isolates with highest motility and lowest levels DNA fragmentation. What known already The selection in Medically Assisted Reproduction should lead fully functional spermatozoa reach fertilization site allow optimal embryo development. These abilities are dependent on specific parameters such as, but not limited to, motility, morphology, Acrosome Index (AI), fragmentation (DFI). At present, routine suboptimal, does replicates physiological processes observed nature consequently may induce damage. new Sperm Sorting technology allows a sperm. However, no comparison other procedures regarding all above-mentioned was reported. design, size, duration Prospective-observational study performed at an University Hospital-IVF Centre between April-November 2022 comparing effect four different various semen parameters. Fifty-two samples minimum 20x106 sperm/ml progressive ≥30% were included. Descriptive statistics reported mean±SD [minimum-maximum] values for each variable. Interclass correlation coefficient (ICC) calculated absolute agreement procedures, where DGC considered standard-of-care. An ICC-value <0.4 indicates low agreement. Participants/materials, setting, methods Unused raw after diagnostic analysis individually split 4 fractions processed immediately by methods: 1) wash (SW), 2) Density Gradient Centrifugation (DGC), 3) Magnetic Activated Cell (MACS, (Miltenyi Biotec)), 4) (MSS) Device (Koek Biotechnology). Each fraction analyzed (AI) (DFI; TUNEL assay protocol). Main results role chance mean age patients 35.9 years (SD ± 6.8). value days abstinence 4.1 Mean rates were: 54.3±10.6% [23-86%], 74.3±11.8% [38-90%], 77.2±12.5% [37-92%] 88.6±4.2% [73-96%] SW, DGC, MACS, MSS, respectively. percentages normal morphology 3.3±2.9% [0-13%], 4.1±3.1% 4.2±3.7% [0-18%], 5.1±3.9% [0-16%] AI 8.5±4.9% [1-20%], 9.7±6.0% [1-30%], 8.7±4.9% [0-19%], 10.8±6.8% [1-30%] DFI 6.2±4.6% [0.8-26.1%], 2.7±3.2% [ 0.2-14%], 2.1±4.3% [0.9-20.8%], 0.2±0.4% [0.0-2.3%] Values referring ICC indicate MSS as only strong disagreement [0.29, 95%CI (-0.2-0.58)] [0.17; (-0.19-0.45)]. No techniques. A further paired confirmed MACS [0.2; (-0.17-0.49) 0.21; (-0.25-0.52)] SW [0.06; (-0.04-0.25) 0.06; (-0.15-0.28)] DFI, Limitations, reasons caution Differences DNA-damage have been smaller techniques if period shorter. Due design experiment, data clinical outcomes available. Wider implications findings highly motile undamaged from unprocessed ideally Plus. Question remains how these compare shorter whether this improves embryological IVF laboratory. Trial registration number NCT05383599